Fascination About hplc column washing procedure
It can be vital that you don't retailer your HPLC column whilst it still is made up of any buffers or ion-pairing brokers. To be certain this, flush 5 column quantityNevertheless, TFA is often really helpful in improving upon retention of analytes such as carboxylic acids, in purposes making use of other detectors like UV-VIS, because it is a reaso